Re: Laboratory safety evaluation of bedinvetmab, a canine anti-nerve growth factor monoclonal antibody, in dogs.

We are writing to express our interest in the article entitled "Laboratory safety evaluation of bedinvetmab, a canine anti-nerve growth factor monoclonal antibody, in dogs", published in the October, 2021 issue of The Veterinary Journal, Volume 276, 105733, by Krautmann and others.

Outcomes of 11 dogs with short radius syndrome treated with acute arthroscopically assisted ulnar shortening.

OBJECTIVE: To describe the short-term outcome of acute arthroscopically assisted ulnar shortening (AUS), to treat short radius syndrome in dogs. STUDY DESIGN: Case series. ANIMALS: Eleven client owned dogs. METHODS: Records of dogs that had undergone AUS for treatment of short radius syndrome were reviewed for inclusion. Reporting data included among others pre- and postoperative radioulnar, humeroradial and humeroulnar distances, lameness scores, surgical times, complications and clinical outcome. RESULTS: Following AUS, radiohumeral articulation was improved in all dogs. Median presurgery radioulnar, humeroradial and humeroulnar values were 4.5, 3.2, and 2.2 mm and were improved with surgery by a median of 3.2, 1.8, and 1.2 mm, respectively. Median surgery time was 140 min. Median time to bone healing was 8 weeks (range: 4-14). Median time to last follow-up was 9 weeks (4-468). Median lameness score (scale 0-4) improved from 2 to 1. No major complications were reported. Short-term clinical outcome was graded by the surgeons as full function in four cases and acceptable function in seven. CONCLUSION AND CLINICAL RELEVANCE: Radiographic and arthroscopic radiohumeral articulation were improved and short-term clinical improvement was documented following AUS in all 11 dogs.

Genome Wide Association Study with Imputed Whole Genome Sequence Data Identifies a 431 kb Risk Haplotype on CFA18 for Congenital Laryngeal Paralysis in Alaskan Sled Dogs.

Congenital laryngeal paralysis (CLP) is an inherited disorder that affects the ability of the dog to exercise and precludes it from functioning as a working sled dog. Though CLP is known to occur in Alaskan sled dogs (ASDs) since 1986, the genetic mutation underlying the disease has not been reported. Using a genome-wide association study (GWAS), we identified a 708 kb region on CFA 18 harboring 226 SNPs to be significantly associated with CLP. The significant SNPs explained 47.06% of the heritability of CLP. We narrowed the region to 431 kb through autozygosity mapping and found 18 of the 20 cases to be homozygous for the risk haplotype. Whole genome sequencing of two cases and a control ASD, and comparison with the genome of 657 dogs from various breeds, confirmed the homozygous status of the risk haplotype to be unique to the CLP cases. Most of the dogs that were homozygous for the risk allele had blue eyes. Gene annotation and a gene-based association study showed that the risk haplotype encompasses genes implicated in developmental and neurodegenerative disorders. Pathway analysis showed enrichment of glycoproteins and glycosaminoglycans biosynthesis, which play a key role in repairing damaged nerves. In conclusion, our results suggest an important role for the identified candidate region in CLP.

Comparison of classic and needle arthroscopy to diagnose canine medial shoulder instability: 31 cases.

This retrospective study compared surgery time, anesthesia time, and costs recorded with classic arthroscopy or needle arthroscopy when diagnosing canine medial shoulder instability. Signalment, examination findings, diagnostics, anesthesia time, surgery time, treatment, invoices, and complications were reported. All cases (classic arthroscopy, 14 cases; needle arthroscopy, 17 cases) were diagnosed with medial shoulder instability. Anesthesia times, surgery times, and invoices were statistically compared for classic and needle arthroscopy (P < 0.05). No significant differences were reported for surgery time (P = 0.13) but existed for anesthesia time (35 minutes shorter with needle arthroscopy; P < 0.0001) and invoice (38% lower with needle arthroscopy; P < 0.0001). No complications were recorded by the time of last direct follow-up, which was at a mean of 12.4 weeks after surgery. Needle arthroscopy offers an alternative, safe technique to reliably diagnose canine medial shoulder instability. Shorter anesthesia times and lower costs to the client may be advantages of needle over classic arthroscopy.

Comparaison de l'arthroscopie classique à l'arthroscopie l'aiguille pour diagnostiquer l'instabilité médiale de l'épaule chez le chien : 31 cas. Cette étude rétrospective a comparé le temps de chirurgie et d'anesthésie ainsi que les coûts engendrés entre une arthroscopie classique et une arthroscopie à l'aiguille lors du diagnostic de l'instabilité médiale de l'épaule chez le chien. Sont rapportés dans cette étude, les commémoratifs, les résultats de l'examen clinique, les diagnostics, le temps d'anesthésie, le temps de chirurgie, le traitement, les coûts et les complications. Le temps d'anesthésie et de chirurgie ainsi que leur coût ont été comparés pour les deux techniques arthroscopie classique et arthroscopie à l'aiguille (P < 0,05) pour tous les cas diagnostiqués avec une instabilité médiale de l'épaule (classique = 14; aiguille = 17). Aucune différence significative n'a été mise en évidence pour la durée de la chirurgie (P = 0,13) à contrario de la durée de l'anesthésie, plus courte (35 min de moins avec la technique à l'aiguille; P < 0,0001) et moins coûteuse (38 % plus économique avec la technique à l'aiguille; P < 0,0001). Aucune complication n'a été enregistrée lors de la dernière consultation de contrôle post-opératoire qui avait lieu en moyenne 12,4 semaines après la chirurgie. Pour diagnostiquer une instabilité médiale de l'épaule, la technique à l'aiguille offre une alternative sûre, avec comme avantages, des durées d'anesthésie plus courtes et des coûts inférieurs pour le client, par rapport à la technique classique.(Traduit par les auteurs).

Orthopedic and ultrasonographic examination findings in 128 shoulders of 64 ultra-endurance Alaskan sled dogs.

OBJECTIVE: To determine the location and periarticular shoulder-muscle-abnormalities detected via orthopedic examinations and ultrasonography in ultra-endurance Alaskan sled-dogs, returned from an ultra-endurance sled-dog-race prior to finishing it. STUDY DESIGN: Prospective clinical study. SAMPLE POPULATION: Sixty-four dogs (128 shoulders). METHODS: Dogs were classified based on clinical evidence of shoulder pain (SP versus control). Orthopedic examination findings, shoulder-abduction-angles (SAA; before- and during-anesthesia), and ultrasonographic findings were recorded. Relationships between orthopedic and ultrasonographic abnormalities were compared. RESULTS: Pain was elicited on 55/128 shoulders; 73 shoulders were pain-free. The most common painful structures included the biceps-tendon (BT; 30%), triceps-muscle (28%), and infraspinatus-muscle (25%). SAA ranged between 25° and 75° among groups, including pain-free shoulders in dogs without lameness. SAA was greater when dogs were anesthetized (46.3° ± 14.0° vs. 47.8° ± 12.0°; p = .03), especially in SP (mean increase of 3.49° ± 8.85°) compared to control (0.03° ± 7.71°, p = .009). Overall, 103 ultrasonographic abnormalities were detected (SP: 44; control: 59). The most common ultrasonographic abnormality was fluid surrounding the biceps tendon, similarly distributed between groups (SP: 39/44; control: 57/59). Most chronic ultrasonographic abnormalities affected the BT (15/103 abnormalities). No associations were detected between ultrasonographic abnormalities and clinical findings. CONCLUSION: Shoulder abduction varied greatly and reached up to 75° in normal joints. Ultrasonographic shoulder-muscle abnormalities were common but did not seem associated with clinical findings. CLINICAL SIGNIFICANCE: Interpretation of shoulder abduction warrants caution, and the presence of fluid around the BT may reflect a physiologic adaptation to racing, rather than a pathologic change in ultra-endurance Alaskan sled-dogs.

Evaluation of a Feline Bone Surrogate and In Vitro Mechanical Comparison of Small Interlocking Nail Systems in Mediolateral Bending.

OBJECTIVE: The aim of this study was to (1) evaluate bending structural properties of a machined short fibre epoxy (SFE) feline bone surrogate (FBS), (2) compare the bending behaviour of small angle-stable interlocking nails (I-Loc; Targon) and locking compression plates (LCP) and (3) evaluate the effect of implant removal on FBS bending strength. METHODS: Part 1: Feline cadaveric femurs (n = 10) and FBS (n = 4) underwent cyclic four-point bending and load to failure. Part 2: Fracture gap FBS constructs (n = 4/group) were stabilized in a bridging fashion with either I-Loc 3 and 4, Targon 2.5 and 3.0, LCP 2.0 and 2.4, then cyclically bent. Part 3: Intact FBS with pilot holes, simulating explantation, (n = 4/group) underwent destructive bending tests. Bending compliance, angular deformation and failure moment (FM) were statistically compared (p < 0.05). RESULTS: Native bone and FBS were similar for all outcome measures (p > 0.05). The smallest and largest bending compliance and angular deformation were seen in the I-Loc 4 and LCP 2.0 respectively (p < 0.05). While explanted Targon FBS had the lowest FM (p < 0.05), I-Loc and LCP constructs FM were not different (p > 0.05). CONCLUSION: The similar bending properties of short fibre epoxy made FBS and native feline femurs suggest that this model could be used for mechanical testing of implants designed for feline long bone osteosynthesis. The I-Loc constructs smaller angular deformation which also suggests that these implants represent a valid alternative to size-matched Targon and LCP for feline fracture osteosynthesis. The significantly lower FM of explanted Targon may increase the risk of secondary fracture following implant removal.

Tibial Apophyseal Percutaneous Pinning in Skeletally Immature Dogs: 25 Cases (2016-2019).

OBJECTIVE: The aim of this study was to report the clinical experience with fluoroscopically guided tibial apophyseal percutaneous pinning (TAPP) for tibial tuberosity avulsion fractures (TTAF). STUDY DESIGN: This is a retrospective case series. MATERIALS AND METHODS: Medical records of skeletally immature dogs (n = 25) were reviewed. The association of pin design [smooth (SP); negatively threaded (NTP)], pin insertion angles, TTAF-type, age and breed with various outcome variables was statistically evaluated. Long-term follow-up was assessed via direct examination, radiographs, questionnaires and videos. RESULTS: Mean age, weight and surgery times were 6.2 months, 9.6 kg and 21 minutes respectively. No major complications occurred among the 19 SP and 6 NTP; no pin broke. Seromas and patellar desmitis rates were higher with SP (11/19; 16/19) versus NTP (0/6; 2/6) (p = 0.02; 0.03). The median horizontal pin cross angles for cases without and with desmitis were 40 and 26 degrees respectively (p = 0.047). Explantation was needed in 5/19 SP and 0/6 NTP cases. The mean tibial plateau angle (TPA) changed significantly between initial (25.6 degrees) and follow-up (18.8 degrees) radiographs (p = 0.0002). Long-term outcome, obtained at a mean of 19.9 months, was excellent in all cases. CONCLUSION: Tibial apophyseal percutaneous pinning can be considered to treat TTAF. Divergent pin placement and using NTP might reduce complications. Tibial plateau angle should be monitored until skeletal maturity has been reached. Long-term outcome post TAPP can be expected to be excellent.

Arthroscopic tenotomy for treatment of biceps tendon luxation in two apprehension police dogs.

CASE DESCRIPTION: A 7.5-year-old 37.8-kg (83.2-lb) sexually intact male German Shepherd Dog (dog 1) and a 2.6-year-old 28.2-kg (62.0-lb) sexually intact male Dutch Shepherd (dog 2), both apprehension police dogs, were admitted for evaluation of left and right thoracic limb lameness, respectively. CLINICAL FINDINGS: In both dogs, signs of pain were elicited on palpation of the shoulder joint in the affected limb, and a distinct popping of the biceps brachii tendon (BT) was palpable on the craniomedial aspect of the affected joint on flexion and extension and was associated with moderate signs of pain. Biceps brachii tendon luxation (BTL) was diagnosed with dynamic musculoskeletal ultrasonography (both dogs) and MRI (dog 1). TREATMENT AND OUTCOME: Arthroscopic BT release by tenotomy was performed in both dogs. Lameness appeared to have resolved by 8 weeks after surgery and had not recurred by the last follow-up communications at 36 and 9 months after surgery for dogs 1 and 2, respectively. Both dogs successfully returned to their level of work performed before their injury. CLINICAL RELEVANCE: Our findings for the dogs of the present report suggested that arthroscopic BT release could be considered a viable treatment option for BTL in dogs, including police dogs or other high-performance athletic dogs. Although our findings provided encouraging results, further research, best conducted with a multicenter prospective randomized study, would be needed to establish the most reliable treatment of BTL in high-performance athletic or working dogs.

Development of a novel fracture fragment stabilization system for minimally invasive osteosynthesis and in vitro comparison to traditional Kern bone reduction forceps.

OBJECTIVE: To develop and evaluate a novel fracture fragment stabilization system, the Sirius minimally invasive bone reduction handle system (SMH), in an artificial fracture model (FxM) simulating a canine femoral fracture repair with a minimally invasive orthopedic approach. STUDY DESIGN: In vitro experimental study. SAMPLE POPULATION: Synthetic fractured femurs with soft-tissue coverage analog (n = 8). METHODS: The developed SMH consisted of modified Kern forceps connected with existing external skeletal fixation components. Intramedullary Steinman pin placement with the SMH or traditional Kern forceps only (KO) was performed by 16 participants in randomized order. Demographics and surgical experience of participants and outcome variables (fragment movement, early/final gap formation, time of procedure, assessed practicability by visual analog scale) were recorded and statistically evaluated. RESULTS: The SMH was more difficult and took longer to assemble (P = .031 and P = .008); SMH resulted in a smaller final reduction gap (P = .008). More surgical experience resulted in faster surgery times (R2 = 0.766) but was not correlated with final fracture gap formation. CONCLUSION: The SMH was associated with reduced fragment gap formation in this simulated setting. CLINICAL SIGNIFICANCE: The SMH may be helpful for maintaining reduction of femoral fractures in dogs. Additional studies of the SMH should be conducted to fully assess the effectiveness and practicality in clinical cases.

Interlocking Nails and Minimally Invasive Osteosynthesis.

Reviews of clinical outcomes led to the foundation of a new approach in fracture management known as biological osteosynthesis. As intramedullary rods featuring cannulations and locking devices at both extremities, interlocking nails are well suited for bridging osteosynthesis. Unique biological and mechanical benefits make them ideal for minimally invasive nail osteosynthesis and an attractive, effective alternative to plating, particularly in revisions of failed plate osteosynthesis. Thanks to a new angle-stable locking design, interlocking nailing indications have been expanded to osteosynthesis of epi-metaphyseal fractures, including those with articular involvement and angular deformities such as distal femoral varus and associated patellar luxations.

Mechanical comparison of two small interlocking nails in torsion using a feline bone surrogate.

OBJECTIVE: To compare the torsional behavior of two small angle-stable interlocking nails (I-Loc and Targon) with that of locking compression plates (LCP). To evaluate the effect of implant removal on the torsional strength of feline bone surrogates. STUDY DESIGN: Experimental. SAMPLE POPULATION: Fracture gap constructs and intact explanted bone surrogates. METHODS: Fracture gap constructs were stabilized with one of six implants (I-Loc 3 and 4, Targon 2.5 and 3.0, LCP 2.0 and 2.4) and then cyclically tested in torsion (n = 4/group). To simulate implant removal, intact surrogates with implant-specific pilot holes were then twisted to failure (n = 4/group). Torsional compliance (TC; °/Nm), angular deformation (AD; °), and failure torque (FT ; Nm) were statistically compared (P < .05). RESULTS: The I-Loc 4 had the smallest TC and AD of all constructs (P < .05). The largest TC (P < .05) was seen with the LCP 2.0. The Targon 2.5 had the largest AD (P < .05) secondary to locking interface slippage. Targon surrogates FT were the lowest of all groups (P < .05). Conversely, there was no difference between the FT of the I-Loc, LCP, and intact surrogates (P > .05). CONCLUSION: We showed that I-Loc nails provided greater torsional stability than size-matched Targon nails and LCPs. Conversely, Targon 2.5 locking interface slippage may jeopardize that construct's stability. Furthermore, the significantly reduced bone surrogate torsional strength provided evidence that the large Targon bolt holes increased the risk of postexplantation iatrogenic fracture. CLINICAL SIGNIFICANCE: Our results provide evidence to conclude that the small I-Loc nails may be valid alternatives to other osteosynthesis options for feline fracture repair.

Successful medical management and rehabilitation of exercise-induced dorsal scapular luxation in an ultramarathon endurance sled dog with magnetic resonance imaging diagnosis of grade II serratus ventralis strain.

Dorsal displacement of the scapula in dogs is rare and often traumatic. This report describes dorsal luxation of the scapula in a sled dog. This case is unique given the injury was sport-related. Magnetic resonance imaging helped direct therapy and monitor healing; and medical management with rehabilitation resulted in full recovery and return to sport. One year after injury, the dog completed both a 482 km and a 1600 km endurance race, placing among the leading teams in the 1600-km race.

Traitement médical réussi d'une luxation scapulaire dorsale aiguë secondaire à la pratique du sport d'endurance chez un chien de traîneau et diagnostic par IRM d'une lésion du m. dentelé ventral. Le déplacement dorsal de la scapula est rare chez le chien et souvent d'origine traumatique. Ce rapport décrit une luxation scapulaire dorsale aiguë chez un chien de traîneau à l'effort. L'intérêt de ce cas tient à sa cause, associé à la pratique de l'activité sportive; à l'analyse des changements en IRM soutenant une thérapie ciblée consistant en un support médical ainsi que d'un programme de rééducation physique, résultant en un rétablissement complet. Un an après cette blessure, le chien termina des courses d'endurance de 482 km et de 1600 km, se classant lors de cette dernière parmi les meilleures équipes de la course.(Traduit par les auteurs).

Congenital laryngeal paralysis in Alaskan Huskies: 25 cases (2009-2014).

OBJECTIVE To characterize congenital laryngeal paralysis (CLP) in Alaskan Huskies. DESIGN Prospective case series. ANIMALS 25 Alaskan Huskies with CLP. PROCEDURES Data were collected for each dog regarding signalment; history; results of physical, orthopedic, neurologic, and laryngeal examinations; esophagraphic findings; treatments; histologic findings; and outcomes. RESULTS Severely affected dogs were profoundly dyspneic at birth or collapsed after brief exercise; less affected dogs reportedly tired easily or overheated with minimal exercise. Mean age at initial onset of clinical signs was 6.4 months. Blue eyes, white facial markings, and oral mucosal tags or tissue bands were noted in 23 (92%), 19 (76%), and 13 (52%) dogs. Neurologic examination revealed signs of mononeuropathy of the recurrent laryngeal nerve but not of polyneuropathy. Histologic examination revealed neurogenic atrophy of the cricoarytenoideus dorsalis muscle but no polyneuropathy. Eight (32%) dogs underwent unilateral cricoarytenoid lateralization, resulting in substantial clinical improvement, including ability to compete in sled dog races. Without surgery, 4 (16%) dogs died of asphyxiation, 10 (40%) had spontaneous improvement of clinical signs (but insufficient improvement to race), and 3 (12%) remained affected. Results of pedigree analysis suggested an autosomal recessive mode of CLP inheritance, with variable penetrance. CONCLUSIONS AND CLINICAL RELEVANCE CLP in the evaluated Alaskan Huskies involved mononeuropathy of the recurrent laryngeal nerves, without polyneuropathy. Most affected dogs had blue eyes, white facial markings, and oral mucosal tags or tissue bands. Given the apparent genetic component to CLP in this breed, we recommend that dogs with these features be prevented from breeding.

Results of a survey of Veterinary Orthopedic Society members on the preferred method for treating cranial cruciate ligament rupture in dogs weighing more than 15 kilograms (33 pounds).

OBJECTIVE To determine which method (lateral fabellotibial suture [LFS], tibial plateau leveling osteotomy [TPLO], tibial tuberosity advancement [TTA], or tightrope-like braided multifilament suture secured with metallic buttons [TR]) Veterinary Orthopedic Society (VOS) members preferred for treating cranial cruciate ligament rupture (CCLR) in dogs weighing > 15 kg (33 lb), identify factors associated with this preference, and assess concerns related to surgical implant material used. DESIGN Cross-sectional study. SAMPLE 187 VOS members. PROCEDURES All registered VOS members received an online survey from June to July 2016. Responses were compiled and evaluated for associations with method preferences and perceived complications. RESULTS Overall response rate was 38.4% (221/575). Respondents had graduated from veterinary school a mean of 23 years prior to survey completion, and collectively they performed approximately 30,000 CCLR surgeries annually. The most commonly preferred method was TPLO (147 [78.6%]), followed by TTA (26 [13.9%]), the LFS procedure (11 [5.9%]), and the TR procedure (3 [1.6%]). The preference for TPLO was independent of board certification or college of training (American, European, or other College of Veterinary Surgeons). Non-board-certified surgeons, including general practitioners, also favored TPLO. The most common perceptions were that titanium implants (used for TTA) were associated with the lowest incidence of major complications, whereas braided multifilament suture (used for the TR procedure) was associated with the highest incidence of major complications. CONCLUSIONS AND CLINICAL RELEVANCE Results suggested that TPLO was preferred for treating CCLR in dogs weighing > 15 kg and that the TR procedure was perceived as having the highest complication rate. With results of this survey in mind, use of the TR procedure should be considered cautiously when treating CCLR.

Perceptions and opinions of pet owners in the United Sates about surgery, pain management, and anesthesia in dogs and cats.

OBJECTIVE: To evaluate pet owners' perceptions and understanding of surgical pain, perioperative pain management, and anesthesia. STUDY DESIGN: Prospective owner survey. SAMPLE POPULATION: Eight veterinary hospitals each provided 200 surveys for distribution to pet owners. METHODS: A survey evaluated owners' perceptions and opinions related to surgical pain, perioperative pain management, anesthesia in dogs and cats, and owner demographics (sex, age, education, employment, previous surgical experience, and pet ownership) in 8 regions of the United States (Alaska, Florida, Hawaii, Massachusetts, Oregon, Ohio, Texas, Washington, DC). Effects of demographics on survey questions were analyzed by using a Cochran-Mantel-Haenszel test. Descriptive statistics and frequency distributions were calculated when applicable. RESULTS: 948/1600 (59.25%) of distributed questionnaires were completed. Owners reported that analgesics were "always needed" more often for surgical procedures than medical conditions. Knowing what to expect during recovery (99%), being informed of procedures and risks (98%), adequate pain management (98%), and having a board-certified anesthesiologist perform anesthesia (94%) were considered "important" or "very important" by owners. The majority of owners agreed that pain affects quality of life (81%), interactions with family and pets (73%), and that declawing is a painful procedure (59%). Older respondents (>46 years), women, owners who have had previous surgery or who have pets that have had previous surgery, and those in health care professions have a better understanding of pain but also expect effective client communication. CONCLUSION: Improving our understanding of pet owners' perceptions and knowledge related to anesthesia, surgery, and pain may lead to improved client education, satisfaction, and compliance with administration of analgesics.

Percutaneous tibial physeal fracture repair in small animals: technique and 17 cases.

OBJECTIVES: To retrospectively describe cases treated via percutaneous tibial physeal fracture repair (PTPFR), using intra-operative fluoroscopy (IFL) or digital radiography (DR). To describe a technique ("spiking"), used to treat tibial tuberosity avulsion fractures. METHODS: Clinical data of 14 dogs and three cats were included. The "spiking" technique was described. RESULTS: Intra-operative fluoroscopy (n = 11) and DR (n = 6) were successfully used in 11 tibial tuberosity avulsion fractures, one combined proximal physeal and tibial tuberosity avulsion fracture, and five distal tibial/fibular physeal fractures. Surgery times ranged from eight to 54 minutes. The "spiking" technique was successfully applied in six tibial tuberosity avulsion fracture cases. Return to function was at a mean (± standard deviation) of 1.9 (± 1.6) weeks. Long-term (>12 months; n = 17) follow-up was available at a mean of 40.6 (± 13.4) months. Major complications consisted of skin irritation from a pin (distal tibia / fibula physeal fracture case; 8 weeks post-PTPFR), and a bilateral grade II medial patella luxation (tibial tuberosity avulsion fracture case; 1.5 years post-PTPFR). One case developed a mild tibial tuberosity avulsion fracture re-avulsion. All conditions in these three cases were not of clinical concern at follow-up and final outcome was graded as good in these and excellent in the other 14 cases. CLINICAL SIGNIFICANCE: Percutaneous tibial physeal fracture repair can be considered as a technique to treat tibial physeal fractures. The "spiking" technique was successfully applied in six dogs. A larger, prospective case series is indicated to provide additional clinical information.

Evaluation of plasma inflammatory cytokine concentrations in racing sled dogs.

In human athletes significant changes in cytokine concentrations secondary to exercise have been observed. This prospective study evaluated the effect of a multi-day stage sled dog race on plasma concentrations of monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor-alpha (TNF-α), interleukin-2 (IL-2), interleukin-6 (IL-6), interleukin-8 (IL-8), and interleukin-10 (IL-10). Samples from 20 dogs were harvested prior to and on days 2 and 8 of an 8-day race. Exercise resulted in significantly decreased TNF-α and IL-8 as well as increases of MCP-1, IL-6, and IL-10 concentrations (P-value between 0.01 and < 0.0001 for all parameters). The proportion of values for IL-2 that were below the detection limit increased from 40% on day 0 to 75% on day 2 and decreased on day 8 to 40% (P = 0.04). Racing sled dogs show cytokine-concentration changes that are different from those in humans.

Évaluation des concentrations plasmatiques de cytokines inflammatoires chez des chiens de traîneau de course. Chez les athlètes humains, des changements importants des concentrations de cytokines secondaires à l'exercice ont été observés. Cette étude prospective a évalué l'effet d'une course de chiens de traîneau par étapes de plusieurs jours sur les concentrations plasmatiques des protéines-1 chimio-attractives des monocytes (MCP-1), du facteur-alpha nécrosant des tumeurs (TNF-α), d'interleukine-2 (IL-2), d'interleukine-6 (IL-6), d'interleukine-8 (IL-8) et d'interleukine-10 (IL-10). Des échantillons ont été prélevés sur 20 chiens avant la course et aux jours 2 et 8 d'une course de 8 jours. L'exercice a produit des valeurs significativement réduites de TNF-α et d'IL-8 ainsi qu'une hausse des concentrations de MCP-1, d'IL-6 et d'IL-10 (la valeur-P entre 0,01 et < 0,0001 pour tous les paramètres). La proportion des valeurs pour IL-2 qui étaient inférieures au seuil de détection a augmenté de 40 % le jour 0 à 75 % le jour 2 et a baissé le jour 8 à 40 % (P = 0,04). Les chiens de traîneau de course montrent des changements de la concentration des cytokines qui sont différents de ceux observés chez les humains.(Traduit par Isabelle Vallières).

Less invasive unilateral arytenoid lateralization: a modified technique for treatment of idiopathic laryngeal paralysis in dogs: technique description and outcome.

OBJECTIVE: To (1) describe a modification of conventional unilateral cricoarytenoid lateralization (UCAL), called less-invasive unilateral cricoarytenoid lateralization (LI-UCAL) for treatment of idiopathic laryngeal paralysis (LP); (2) report clinical outcome of LI-UCAL; and (3) describe the effect of early discharge after surgery. STUDY DESIGN: Retrospective clinical study. ANIMALS: Dogs (n = 22). METHODS: Medical records (January 2009 to October 2011) of dogs diagnosed with idiopathic LP that had LI-UCAL were reviewed. Signalment, clinical signs, laboratory tests, imaging, concurrent medical conditions, information from the anesthesia record, and hospitalization time were documented. Follow-up was obtained by direct examination, questionnaire, and review of medical records from referring veterinarians. RESULTS: Dogs included in the study had variable degrees and duration of respiratory distress before surgery. Median surgery time was 32 minutes. Median hospitalization was 6 hours, and median follow-up was 427 days. Long-term respiratory function, as reported by owners at last follow-up, improved in 95.5% of dogs after surgery; exercise tolerance improved by 90%. Major complications included death secondary to aspiration pneumonia (9%), aspiration pneumonia from which dogs recovered within 3 days after administration of antimicrobials (9%), and development of right-sided laryngeal collapse that required right sided LI-UCAL (4.5%). At long-term follow-up, 95.5% of owners were satisfied with the surgical outcome. CONCLUSION: LI-UCAL is a feasible surgical technique and resulted in improvement of clinical signs related to LP. Calm recoveries without adverse effects such as respiratory distress were associated with early discharge. LI-UCAL could be considered an alternative to conventional UCAL.

Effect of screw insertion torque on mechanical properties of four locking systems.

OBJECTIVE: To evaluate the effect of screw insertion torque on the mechanical properties of four 3.5 mm locking systems: New Generation Devices (NGD), Securos (PAX), Synthes (SYN), and Veterinary Orthopedic Implants (VOI). STUDY DESIGN: In vitro mechanical study. METHODS: Screws were inserted at 1.5 Nm, 2.5 Nm, and 3.5 Nm torques, using dedicated drill guides and a calibrated torque screwdriver. Locking mechanisms were tested under shear loading conditions using a custom-design probe. Tests were conducted under displacement control until failure. Load to failure and interface stiffness were compared between and within groups using a two-factor ANOVA (P < .05). Failure modes were described. RESULTS: The SYN group had significantly greater mechanical properties compared to all other groups at any given insertion torque. Insertion torque had a significant effect on the PAX group, increasing the load to failure by 126% when increasing the insertion torque from 1.5 Nm to 3.5 Nm. Insertion torque had no significant effect on the mechanical properties of the VOI group and limited effect on the NGD and SYN groups. Single failure mode, by screw head decoupling or screw shaft fracture, occurred in the SYN and VOI groups, respectively. In contrast, NGD and PAX systems failed through a combination of screw decoupling and/or bending. CONCLUSIONS: Regardless of insertion torque, the SYN locking mechanism showed the highest failure loads, interfacial stiffness as well as consistent failure mode. These findings highlight the dependability of this system. When using the PAX system an insertion torque of at least 2.5 Nm should be recommended.